https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Soil phosphatase assay to evaluate arsenic toxicity should be performed at the soil's actual pH https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50272 Wed 12 Jul 2023 14:11:21 AEST ]]> Respecting catalytic efficiency of soil arylsulfatase as soil Sb contamination bio-indicator by enzyme kinetic strategy https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52779 Tue 14 Nov 2023 14:21:49 AEDT ]]> Differences in the response of soil dehydrogenase activity to Cd contamination are determined by the different substrates used for its determination https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33556 Tue 03 Sep 2019 18:31:47 AEST ]]> Soil mineral alters the effect of Cd on the alkaline phosphatase activity https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41360 Tue 02 Aug 2022 14:31:24 AEST ]]> The effect of arsenic on soil intracellular and potential extracellular β-glucosidase differentiated by chloroform fumigation https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39536 Mon 08 Aug 2022 11:27:21 AEST ]]> The accuracy in the assessment of arsenic toxicity using soil alkaline phosphatase depends on soil water contents https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41831 -1 and 400 mg kg-1), F1 and F2 transformed to F4 and F5. Meanwhile, soil moisture had a significant influence on soil ALP activity. Soil ALP activity under 110% WHC was smaller than the activity of soil ALP under 35% WHC. The variation partitioning analysis (VPA) showed that soil moisture contributed 63.19% for this effect on soil ALP activity and was identified as the dominant factor. The value of ED 10 indicated that the As toxicity under 35% WHC was greater than that under 110% WHC. This could be due to that the excessive moisture content depressed soil ALP activity. Therefore, it is important to consider soil moisture content while assessing the As toxicity to soil ALP.]]> Fri 26 Aug 2022 11:21:16 AEST ]]> Catalytic efficiency is a better predictor of arsenic toxicity to soil alkaline phosphatase https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32983 max) and Michaelis constant (Km) in unpolluted soils were 0.012-0.267 mM h-1 and 1.34-3.79 mM respectively. The competitive inhibition constant (Kic) was 0.17-0.70 mM, which was lower than Km, suggesting higher enzyme affinity for As than for substrate. The ecological doses, ED₁₀ and ED₅₀ (concentration of As that results in 10% and 50% inhibition on enzyme parameter) for inhibition of catalytic efficiency (Vmax/Km) were lower than those for inhibition of enzyme activity at different substrate concentrations. This suggests that the integrated kinetic parameter, catalytic efficiency is substrate concentration independent and more sensitive to As than ALP activity. Thus, catalytic efficiency was proposed as a more reliable indicator than ALP activity for risk assessment of As pollution.]]> Fri 17 Aug 2018 15:44:20 AEST ]]> Soil enzyme kinetics indicate ecotoxicity of long-term arsenic pollution in the soil at field scale https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40598 Fri 15 Jul 2022 11:21:12 AEST ]]>